Device

Part:BBa_K812013:Design

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-18)


GFP-AID OsTirI polysistronic system for auxin detection in tadpole


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1990
    Illegal NotI site found at 1844
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3205
    Illegal XhoI site found at 1913
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1612
    Illegal NgoMIV site found at 1721
    Illegal AgeI site found at 1021
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1406
    Illegal BsaI site found at 1636


Design Notes

This sequence has been assembled using the golden-gate method. Therefore, the biobrick extension are slightely modified, but keep the EcoRI XbaI SpeI PstI system.


Source

This device is the reassembly of the parts obtained by PCR in the PNHK60 plasmid from the riken registry.

References